Gene description for FN1 |
Gene name |
fibronectin 1 |
Gene symbol |
FN1 |
Other names/aliases |
CIG ED-B FINC FN FNZ GFND GFND2 LETS MSF SMDCF |
Species |
Homo sapiens |
Experiment description of studies that identified FN1 in Breast cancer cells |
1
|
Experiment ID | 210 |
Identified molecule | protein
|
Extracellular vesicle type | Microvesicles |
MISEV | EVs |
Identification method | Western blotting
|
PubMed ID |
21368175
|
Organism | Homo sapiens |
Experiment description | Cancer cell-derived microvesicles induce transformation by transferring tissue transglutaminase and fibronectin to recipient cells |
Authors | Antonyak MA, Li B, Boroughs LK, Johnson JL, Druso JE, Bryant KL, Holowka DA, Cerione RA |
Journal name |
Proc Natl Acad Sci U S A
|
Publication year | 2011 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 |
Isolation/purification methods | Differential centrifugation Filtration |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Immunofluorescent microscopy Scanning electron microscopy Mass spectrometry [4000 Q Trap] |
EV-TRACK |
-
|
|
|
2
|
Experiment ID | 575 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry [LTQ]
|
PubMed ID |
27894104
|
Organism | Homo sapiens |
Experiment description | Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. |
Authors | Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. |
Journal name |
Oncotarget
|
Publication year | 2016 |
Sample | Breast cancer cells |
Sample name | BT549 |
Isolation/purification methods | Differential centrifugation Polymer-based precipitation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [LTQ] |
EV-TRACK |
-
|
|
|
3
|
Experiment ID | 577 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry [LTQ]
|
PubMed ID |
27894104
|
Organism | Homo sapiens |
Experiment description | Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. |
Authors | Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. |
Journal name |
Oncotarget
|
Publication year | 2016 |
Sample | Breast cancer cells |
Sample name | MCF7 |
Isolation/purification methods | Differential centrifugation Polymer-based precipitation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [LTQ] |
EV-TRACK |
-
|
|
|
4
|
Experiment ID | 579 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry [LTQ]
|
PubMed ID |
27894104
|
Organism | Homo sapiens |
Experiment description | Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. |
Authors | Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. |
Journal name |
Oncotarget
|
Publication year | 2016 |
Sample | Breast cancer cells |
Sample name | MDA-MB-468 |
Isolation/purification methods | Differential centrifugation Polymer-based precipitation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry [LTQ] |
EV-TRACK |
-
|
|
|
5
|
Experiment ID | 1029 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
26378940
|
Organism | Homo sapiens |
Experiment description | Redefining the Breast Cancer Exosome Proteome by Tandem Mass Tag Quantitative Proteomics and Multivariate Cluster Analysis. |
Authors | Clark DJ, Fondrie WE, Liao Z, Hanson PI, Fulton A, Mao L, Yang AJ. |
Journal name |
Anal Chem.
|
Publication year | 2015 |
Sample | Breast cancer cells |
Sample name | SKBR3B - 100 K pellet |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry Western blotting |
EV-TRACK |
EV150004: EV-METRIC:67%, 38%
|
|
|
6
|
Experiment ID | 1254 |
Identified molecule | protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | EVs |
Identification method | Mass spectrometry
|
PubMed ID |
24468161
|
Organism | Homo sapiens |
Experiment description | Molecular characterization of exosome-like vesicles from breast cancer cells. |
Authors | Kruger S, Abd Elmageed ZY, Hawke DH, Wör PM, Jansen DA, Abdel-Mageed AB, Alt EU, Izadpanah R. |
Journal name |
BMC Cancer.
|
Publication year | 2014 |
Sample | Breast cancer cells |
Sample name | MCF7 |
Isolation/purification methods | Differential centrifugation Filtration Ultracentrifugation Density gradient centrifugation |
Flotation density | - |
Molecules identified in the study | Protein miRNA |
Methods used in the study | Mass spectrometry Microarray RT-PCR Western blotting |
EV-TRACK |
EV140357: EV-METRIC:22%
|
|
|
7
|
Experiment ID | 1957 |
Identified molecule | protein
|
Extracellular vesicle type | Microvesicles |
MISEV | Large EVs |
Identification method | Western blotting
|
PubMed ID |
36475545
|
Organism | Homo sapiens |
Experiment description | Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis |
Authors | Schwager SC, Young KM, Hapach LA, Carlson CM, Mosier JA, McArdle TJ, Wang W, Schunk C, Jayathilake AL, Bates ME, Bordeleau F, Antonyak MA, Cerione RA, Reinhart-King CA. |
Journal name |
Elife
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - weakly migratory (MDA- ) |
Isolation/purification methods | Centrifugation Filtration |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectometry |
EV-TRACK |
-
|
|
|
8
|
Experiment ID | 1958 |
Identified molecule | protein
|
Extracellular vesicle type | Microvesicles |
MISEV | Large EVs |
Identification method | Western blotting
|
PubMed ID |
36475545
|
Organism | Homo sapiens |
Experiment description | Weakly migratory metastatic breast cancer cells activate fibroblasts via microvesicle-Tg2 to facilitate dissemination and metastasis |
Authors | Schwager SC, Young KM, Hapach LA, Carlson CM, Mosier JA, McArdle TJ, Wang W, Schunk C, Jayathilake AL, Bates ME, Bordeleau F, Antonyak MA, Cerione RA, Reinhart-King CA. |
Journal name |
Elife
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - highly migratory (MDA+) |
Isolation/purification methods | Centrifugation Filtration |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectometry |
EV-TRACK |
-
|
|
|
9
|
Experiment ID | 1678 |
Identified molecule | Protein
|
Extracellular vesicle type | Exosomes |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
34108659
|
Organism | Homo sapiens |
Experiment description | Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors | Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
Journal name |
Nat Cell Biol
|
Publication year | 2021 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - Exo-rich fractions 7-10 pooled |
Isolation/purification methods | Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectrometry |
EV-TRACK |
-
|
|
|
10
|
Experiment ID | 1679 |
Identified molecule | Protein
|
Extracellular vesicle type | Exosomes |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
34108659
|
Organism | Homo sapiens |
Experiment description | Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
Authors | Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
Journal name |
Nat Cell Biol
|
Publication year | 2021 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 - Exo-rich fractions 1-6 pooled |
Isolation/purification methods | Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectrometry |
EV-TRACK |
-
|
|
|
11
|
Experiment ID | 1758 |
Identified molecule | Protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
35918900
|
Organism | Homo sapiens |
Experiment description | Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
Authors | Bauzá-Martinez J, Armony G, Pronker MF, Wu W. |
Journal name |
J Extracell Vesicles
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectometry Western blotting |
EV-TRACK |
-
|
|
|
12
|
Experiment ID | 1758 |
Identified molecule | Protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
35918900
|
Organism | Homo sapiens |
Experiment description | Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
Authors | Bauzá-Martinez J, Armony G, Pronker MF, Wu W. |
Journal name |
J Extracell Vesicles
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectometry Western blotting |
EV-TRACK |
-
|
|
|
13
|
Experiment ID | 1759 |
Identified molecule | Protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
35918900
|
Organism | Homo sapiens |
Experiment description | Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
Authors | Bauzá-Martinez J, Armony G, Pronker MF, Wu W. |
Journal name |
J Extracell Vesicles
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | LM2 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectometry |
EV-TRACK |
-
|
|
|
14
|
Experiment ID | 1760 |
Identified molecule | Protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
35918900
|
Organism | Homo sapiens |
Experiment description | Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
Authors | Bauzá-Martinez J, Armony G, Pronker MF, Wu W. |
Journal name |
J Extracell Vesicles
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectometry |
EV-TRACK |
-
|
|
|
15
|
Experiment ID | 1760 |
Identified molecule | Protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
35918900
|
Organism | Homo sapiens |
Experiment description | Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
Authors | Bauzá-Martinez J, Armony G, Pronker MF, Wu W. |
Journal name |
J Extracell Vesicles
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectometry |
EV-TRACK |
-
|
|
|
16
|
Experiment ID | 1761 |
Identified molecule | Protein
|
Extracellular vesicle type | Extracellular vesicles |
MISEV | Small EVs |
Identification method | Mass spectrometry
|
PubMed ID |
35918900
|
Organism | Homo sapiens |
Experiment description | Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL) |
Authors | Bauzá-Martinez J, Armony G, Pronker MF, Wu W. |
Journal name |
J Extracell Vesicles
|
Publication year | 2022 |
Sample | Breast cancer cells |
Sample name | LM2 |
Isolation/purification methods | Differential centrifugation Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Mass spectometry |
EV-TRACK |
-
|
|
|
17
|
Experiment ID | 3350 |
Identified molecule | protein
|
Extracellular vesicle type | Supermeres |
MISEV | EV-like nanoparticles |
Identification method | Mass spectrometry
|
PubMed ID |
34887515
|
Organism | Homo sapiens |
Experiment description | Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
Authors | Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. |
Journal name |
Nat Cell Biol
|
Publication year | 2021 |
Sample | Breast cancer cells |
Sample name | MDA-MB-231 |
Isolation/purification methods | Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation |
Flotation density | - |
Molecules identified in the study | Protein |
Methods used in the study | Western blotting Mass spectrometry ELISA |
EV-TRACK |
-
|
|
|